| Antigen | Bacterial GST (Schistosoma japonicum, ~27 kda) was expressed in E. coli and purified (>97%). Purified GST was injected into rabbits to produce high titer polyclonal antibodies. Antibodies were affinity purified (IgG).
Affinity purified ratbbit anti-GST IgG was coupled to highly purified calf intestinal alkaline phosphatase, AP (Cat # GST11-AP) using glutaraldehyde method. Antibody:AP molar rati is ~1-1.5),. Antibody concn. Is ~1 mg/ml. Anti-GST-AP conjugate is supplied in 05M Tris, pH 8 containing 1% BSA, 1 mM MgCl2 and 05% sodium azide |
| Form | Antibody Pure IgG |
| Product Type | Antibodies |
| SpCrossreactivity | Anti-GST emzyme conjugates (HRP/AP) recognize both the native and denatured GST or the fusion proteins. No reactivity is observed with mammalian (rabbit, mouse, rat, and human) GSTs. |
| SpeciesReactivity | E. coli |
| SugNegativeControl | Non-immune Rabbit IgG Cat# 20009-1 |
| SugSecAntibodies | http://4adi.com/commerce/ccc2480-secondary-antibodies-rabbit.htm |