| Antigen | #NITT12-C (10-20 blots) has a mixture of mol. Wt calibrated proteins (superoxide dismutase, ~16 kDa, occasionally a dimmer of ~32 kDa may also be seen; BSA ~66 kDa; and muscle myosin ~215 kDa) that were nitrated with peroxynitrite for use in Western blotting using the polyclonal anti-nitrotyrosine. Not recommended for use with monoclonal anti-nitrotyrosine. NITT12-C is supplied in SDS-PAGE sample buffer (reduced) for Western. Load 10 ul/lane of NITT12-C for good visibility with antibody Cat # NITT12-A or # NITT13-S |