| Antigen | HNE-BSA conjugate (cat #HNE12-C) is supplied in 100 ul of SDS-PAGE sample buffer (reduced) and it is only suitable for Western blot +ve control. It cannot be used for ELISA etc. Load approx 10 ul/lane for blotting with antibodies (Cat # HNE11-S).. Loading volume may be adjusted depending upon the intensity of the band under actual experimental condition. BSA-HNE conjugate is approx. ~65-70 kda. Other aggregated bands may be visible as well |