| Antigen | 14-aa peptide from rat Glut-7; (protein Accession #S24344) Designation (GT71-P, control peptide) conjugated to KLH; Epitope location~ C-terminal, Cytoplasmic domain |
| Form | Antigen/blocking Peptide |
| Product Type | Peptide |
| SpCrossreactivity | Rat GT71 sequence is 64% conserved in human. Glut-7 and Glut-2 sequences are 68% identical in rats and show similar secondary and tertiary structures. However, Glut-7 contains an extra 6 amino acid (KKMKND) at the C-terminal end. This sequence is very similar to the last 6 amino acids of some of the mircosomal UDP-glucoronosyltransferase isoenzymes (see refs. 1 and other refs. Cited therein). The KK-K-- has been considered motif for the retention of transmembrane proteins (3) and is not found in Gluts 1-5. The crossreactivity of anti-rat GT71 antibody to other proteins with an endoplasmic reticulum motif and species crossreactivity has not been determined. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity |
| SpeciesReactivity | Rat |
| SugApplications | Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. |
