| Antigen | A DNA sequence encoding the signal peptide from human CD33 joined with amino acid residues 20-232 of mouse noggin was fused to the Fc region of human IgG, via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NSO. The recombinant mature mouse noggin/ Fc is a disulphide-linked homodimeric protein. Based on N-terminal sequencing, the reduced mouse noggin/Fc monomer has a calculated mol. mass of ~50kD. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60kD protein in SDS-PAGE under reducing conditions. |