| Antigen | Human Acrp30 (1-244 aa) was expressed as C-terminal his-tagged protein in mouse myeloma cells (NSO) and purified (>90%) using nickel-columns.. Recombinant mature protein (19-244 aa, ~25.4 kda) migrates at ~36 kda due to glycosylation. The endotoxin levels were found to be minimal (<1 EU/1 ug protein). |