Catalog# Size Unit Price Quantity
BACE-SW 100 ug $125.00

Human BACE/Asp2 substrate (wild type) APP590-603

Antigen¿-Secretase Substrate (Swedish) Human APP590-603 Acetyl-EISEVNL ¿ DA EFR HD-amide
FormPurified Peptide
Product TypePeptide
SpCrossreactivityPurified BACE/Asp2 cleaves APP and synthetic APP peptide substrates at the ¿-secretase site, and the rate of cleavage is increase 10-fold by a Swedish type mutation associated with early onset of Alzheimer?s disease. BACE has an acidic optimum (pH 4). Typically, purified BACE/Asp2 prepared by membrane solubilization in 25 mM Tris, pH 8, containing 50 mM ¿-octylglucoside retained the enzyme activity. Enzymatic reactions are performed in 50 mM 2-(N-morpholino)ethane-sulfomate, pH 5.5, containing 70 uM peptide substrate and the reaction performed at 37oC [see details in Yan et al (1999) and Lin et al (1999)]. Reaction products are then analyzed by reverse phase HPLC.
SpeciesReactivityHuman
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