Custom Peptide Synthesis & Antibodies

Alpha Diagnostic Intl. Inc. (ADI) has been providing custom peptide and antibody services for the last 20 years. Our products and services have been received and appreciate by Scientists from almost every research institution and company not only in the USA and Canada but also from highly international institution.  ADI greatly appreciates this level of confidence and we will continue to improve and excel and set even higher standards.  Most companies rely on some kind of testimonial or offer of the lowest prices, ADI offers its track record, highest quality and service, and competitive prices.

ADI has ~25 years of track record.
 
ADI offers the most comprehensive 
Protein Sequence and Structure Analyses (PSSA) not only to find antigenic peptides but also to learn a lot more about the protein structure, intracellular location, and potential functions.  There is no charge for this service. It will be extremely hard to expect this level of expertise from other companies even for a fee.
 
ADI is the most cited company for custom peptide and antibodies. You will find “Alpha Diagnostic Intl” name in most issues of Nature, Science, Cell, J. Biol. Chem., Proc. Natl. Acad. Sci., and other top-ranked journals. ADI has posted a list of  
several 100 publications to document our custom services. Our testimonials are these citations.
 
ADI offers the most efficient execution of ‘your ideas and experimentations’ with its in-house USDA and NIH/OLAW approved animal research facility.
 
ADI offers the highest level of quality and service for very competitive price.  ADI will meet or exceed a quote for identical service from any other US company.

ADI offers custom peptide synthesis, conjugation, antibodies, and purification in a single facility. This will not only save valuable time but it is also most economical. We can conjugate the supplied peptide to carrier proteins and make antibodies or make antibodies to a conjugate supplied by researchers. You can request only the peptide synthesis or have the peptide conjugated to carrier proteins. We are willing to offer as much help as necessary.

Custom Peptide Synthesis Only Please click over the links for more info

Custom Antibodies to Ready-to-Inject Antigens/proteins (Proteins, Peptide-Protein Conjugates, and Gels).

Custom Antibodies to Phosphopeptides

Custom Antibodies to Acetylated (lysine)

Custom Peptide Synthesis, Conjugation, and Anti-Peptide Antibodies.

Anti-Peptide Antibody package is designed to be economical and includes the following:

·         Peptide synthesis (HPLC, Mass Spec, Scale 10-15 mg; 70-85% purity)
 

·         Supply of free peptide (~ 5 mg is used for antibody production and ELISA and the rest is supplied to the researcher).
 

·         Conjugation of peptide to carrier protein (KLH, BSA, ovalbumin, etc.).
 

·         Antibody production in 2 rabbits (or 2 G. Pigs or 2 Chickens, extra) using std. 63 days protocol.
 

·         ELISA titration of each antibody on first bleed.
 

·         Approximate duration of projects (from peptide order to first bleed): 9-11 weeks depending upon the peptide length.
 

·         Free Services:Selection of Antigenic Peptides, BLAST searches, Identification of Signal peptide, Transmembrane domains, Sequence alignments, and other common structural motifs (Click here for details)

Peptide Synthesis and Antibody Ordering Information (Click here to open the fax order form)

Peptide Length

Std Package
Cat #

With Peptide Upgrade
Cat #

With Affinity Purification
Cat #

With Peptide Amount Upgrade & Aff. Pur.
Cat #

Up to 20 AA

ABPEP-20

ABPEP-20U

ABPEP-20A

ABPEP-20UA

Up to 25 AA

ABPEP-25

ABPEP-25U

ABPEP-25A

ABPEP-25UA

Up to 30 AA

ABPEP-30

ABPEP-30U

ABPEP-30A

ABPEP-30UA

Summary of Services Included in Various Packages:

Items/
Services

Std Package

With Peptide Upgrade

With Affinity Purification

With Peptide Amount Upgrade & Aff. Pure

Free Protein sequence
& antigenicity analyses

 Yes

Yes

Yes

Yes

Peptides
(70-85% pure, mass spec, hplc)

5-10 mg total

10-20 mg total

5-10 mg total

10-20 mg total

Supply of free peptide

5 mg

~10-15 mg

~5 mg

~10 mg

Peptide Conjugation 
(couple ~ 5 mg to KLH etc)

Yes

Yes

Yes

Yes

Animals (2 rabbits)
& 63-days immunization program

Yes

Yes

Yes

Yes

Pre-immune bleeds(2-5 ml/animal)

Yes

Yes

Yes

Yes

Production Bleeds
(15-20 mls/animal) total 70-80 mls

Yes

Yes

Yes

Yes

ELISATesting of 1st bleeds

Yes

Yes

Yes

Yes

Affinity Purification (includes coupling of ~ 5 mg peptide to Sepharose, purification of 1 bleed, ELISA testing of bleeds and supply of the affinity column)

 

 

Yes

Yes

Antibody Production Guarantee
(Minimum antibody titer by ELISA ~1K or repeat the project at no cost
(see details below)

Yes

Yes

Yes

Yes

All projects include 2 rabbits, but we can substitute them with 2 G. Pig/Hamsters/or Chicken at no extra charge. Goat and sheep will incur additional charges. We can modify the std immunization and bleed protocols and include more animals if requested. Other animals, not included above, can be provided as well. Please contact ADI for current prices and availability. Std 63 days protocol include up to 5 injections (i.m. and s.c. at multiple sites at 15 days intervals); 1 preimmune bleed (~2 ml per animal), 2 immune bleeds (std. bleeds at weeks 7 and 9; ~15-20 mls per rabbit). All projects may be extended on a monthly basis, for a nominal fee, to collect more antiserum (2 bleeds per month). Please inform us prior to the conclusion of std protocol if you wish to extend the projects. Please consult Some Commonly Asked Questions About Custom Peptide And Antibody Servicesfor bleed volume in other species and optional services.

Peptide Amounts Upgrades: Standard package includes about ~10-20 mg of >90% pure peptide. This is normally sufficient to produce antibodies and have some free peptide (at least 5-10 mg) for additional experiments. However, if affinity purification (need approx. 5-10-mg peptide) is intended or needed, it is a good idea to Upgrade Amounts synthesis (synthesize 20-40 mg of 70-85% pure).

Affinity purification this package typically includes:
Coupling of peptide (up to 10 mg to Sepharose), purification of any 1 bleed of ~ 15 ml or a pool of antiserum up to 15 ml; ELISA testing of both unpurified and affinity pure antibodies, and supply of affinity column We also monitor the antibody activity in the unbound fraction to make sure antibodies are not overloaded and wasted. Ideally, we need approx. ~10 mg of antigen or peptide for making the affinity matrix. This package (Cat # AFFI-1; $695) can also be ordered separately.

Note : Affinity purification is normally scheduled on 2nd bleed (pool of sera from both rabbits) unless antibody titer is poor in the first and 2nd bleed. However, user may elect to delay affinity purification or use just 1 rabbit serum. We must be informed prior to the second bleed.

ELISA: ADI uses proprietary techniques and buffer solutions to coat free peptides on ELISA plates. Therefore, antibodies to the carrier protein are not detected. We test individual preimmune and immune serum at 4 dilutions (1:100, 1:1K, and 1:10K, 1:100K). A detailed ELISA report is provided with every test regarding antibody titer of each animal. Antibody titer reported in our ELISA report refers only to anti-peptide antibodies. Complete instructions of performing ELISA and reagents used are given in ELISA Procedure.

190123A

Catalog# Name Product Type Data Sheet Size Price
ABPEP-20GPA Custom Anti-Peptide antibodies (peptide synth. ~20-aa, conjug., 2 G. Pig, ELISA & Aff. Purif.) Service 1 set 1990.0
ABPEP-20GP Custom Anti-Peptide antibodies (peptide synthesis up to 20-aa, conjugation, 2 G. pig, ELISA) Service 1 set 1395.0
ABPEP-20CHA Custom Anti-Peptide antibodies (peptide synth. ~20-aa, conjug., 2 Chicken, ELISA & IgY from 6 egg yolk Aff. Purif.) Service 1 set 1990.0
ABPEP-20CH Custom Anti-Peptide antibodies (peptide synthesis up to 20-aa, conjugation, 2 Chicken, ELISA & IgY from 6 egg yolk) Service 1 set 1395.0
AFFI-I Custom Affinity Purification of antibodies (Coupling of antigen to Agarose, Ab purification (10 ml), ELISA, and affinity column Others 1 set 695.0
ABPEP-20SA Custom Anti-Peptide antibodies ( up to 20-aa, conjugation, 1 sheep, ELISA, aff pure); Add $895 for additional sheep Others 1 set 2390.0
ABPEP-20SUA Custom Anti-Peptide antibodies ( up to 20-aa, peptide amounts upgrade; conjugation, 1 sheep, ELISA, aff pure) Others 1 set 2590.0
ABPEP-20GUA Custom Anti-Peptide antibodies ( up to 20-aa, peptide amounts upgrade; conjugation, 1 goat, ELISA, aff pure) Others 1 set 2590.0
ABPEP-20GA Custom Anti-Peptide antibodies ( up to 20-aa, conjugation, 1 goat, ELISA, aff pure); Add $895 for additional goat Others 1 set 2390.0
AFFI-2 Custom Conjugation of peptides/proteins to Agarose (up to 10 mg antigen) Service 1 set 395.0
ABPEP-30UA Custom Anti-Peptide antibodies (peptide synth. ~30-aa, conjug., 2 rabbits, ELISA; with peptide upgrade and Aff. Purif.) Service 1 set 2690.0
ABPEP-30U Custom Anti-Peptide antibodies (peptide synth. up to 30-aa, conjug., 2 rabbits, ELISA; with peptide amounts upgrade) Service 1 set 2095.0
ABPEP-30A Custom Anti-Peptide antibodies (peptide synth. ~30-aa, conjug., 2 rabbits, ELISA & Aff. Purif.) Service 1 set 2390.0
ABPEP-30 Custom Anti-Peptide antibodies (peptide synthesis up to 30-aa, conjugation, 2 rabbits, ELISA) Service 1 set 1795.0
ABPEP-25UA Custom Anti-Peptide antibodies (peptide synth. ~25-aa, conjug., 2 rabbits, ELISA; with peptide upgrade and Aff. Purif.) Service 1 set 2490.0
ABPEP-25U Custom Anti-Peptide antibodies (peptide synth. up to 25-aa, conjug., 2 rabbits, ELISA; with peptide amounts upgrade) Service 1 set 1895.0
ABPEP-25A Custom Anti-Peptide antibodies (peptide synth. ~25-aa, conjug., 2 rabbits, ELISA & Aff. Purif.) Service 1 set 2240.0
ABPEP-25 Custom Anti-Peptide antibodies (peptide synthesis up to 25-aa, conjugation, 2 rabbits, ELISA) Service 1 set 1645.0
ABPEP-20UA Custom Anti-Peptide antibodies (peptide synth. ~20-aa, conjug., 2 rabbits, ELISA; with peptide upgrade and Aff. Purif.) Service 1 set 2290.0
ABPEP-20U Custom Anti-Peptide antibodies (peptide synth. up to 20-aa, conjug., 2 rabbits, ELISA; with peptide amounts upgrade) Service 1 set 1695.0
ABPEP-20SU Custom Anti-Peptide antibodies (peptide up to 20-aa, peptide amounts upgrade; conjugation, 1 sheep, ELISA) Service 1 set 1995.0
ABPEP-20S Custom Anti-Peptide antibodies (peptide up to 20-aa, conjugation, 1 sheep, ELISA); Add $895 for additional sheep Service 1 set 1795.0
ABPEP-20GU Custom Anti-Peptide antibodies (peptide up to 20-aa, peptide amounts upgrade; conjugation, 1 goat, ELISA) Service 1 set 1995.0
ABPEP-20G Custom Anti-Peptide antibodies (peptide up to 20-aa, conjugation, 1 goat, ELISA); Add $895 for additional goat Service 1 set 1795.0
ABPEP-20A-2 Custom Anti-Peptide antibodies 2 peptides (upto 20-aa, synthesis, 2 conjugation, 2 rabbits, 2 ELISA & 2 Aff. Purif.) Service 1 set 3285.0
ABPEP-20A-1 Custom Anti-Peptide antibodies, 2 peptides (upto 20-aa; synthesis,2 conjugation, 2 rabbits, 2 ELISA & 1 Aff purif) Service 1 set 2690.0
ABPEP-20A Custom Anti-Peptide antibodies (peptide synth. ~20-aa, conjug., 2 rabbits, ELISA & Aff. Purif.) Service 1 set 2090.0
ABPEP-20-2 Custom Anti-Peptide antibodies, 2 peptides (upto 20-aa; synthesis, 2 conjugation, 2 rabbits, 2 ELISA) Service 1 set 2095.0
ABPEP-20 Custom Anti-Peptide antibodies (peptide synthesis up to 20-aa, conjugation, 2 rabbits, ELISA) Service 1 set 1495.0
ASCITE-1 Custom Production of Ascites in Balb/c mice (min 20 mice @$30/mice plus; $300 per cell line for cell culture) Service 1 set 900.0