- Home
- Custom peptides & Antibodies
- Custom Peptide Synthesis & Antibodies
Custom Peptide Synthesis & Antibodies
Alpha Diagnostic Intl. Inc. (ADI) has been providing custom peptide and antibody services for the last 20 years. Our products and services have been received and appreciate by Scientists from almost every research institution and company not only in the USA and Canada but also from highly international institution. ADI greatly appreciates this level of confidence and we will continue to improve and excel and set even higher standards. Most companies rely on some kind of testimonial or offer of the lowest prices, ADI offers its track record, highest quality and service, and competitive prices.
ADI has ~25 years of track record.
ADI offers the most comprehensive Protein Sequence and Structure Analyses (PSSA) not only to find antigenic peptides but also to learn a lot more about the protein structure, intracellular location, and potential functions. There is no charge for this service. It will be extremely hard to expect this level of expertise from other companies even for a fee.
ADI is the most cited company for custom peptide and antibodies. You will find “Alpha Diagnostic Intl” name in most issues of Nature, Science, Cell, J. Biol. Chem., Proc. Natl. Acad. Sci., and other top-ranked journals. ADI has posted a list of several 100 publications to document our custom services. Our testimonials are these citations.
ADI offers the most efficient execution of ‘your ideas and experimentations’ with its in-house USDA and NIH/OLAW approved animal research facility.
ADI offers the highest level of quality and service for very competitive price. ADI will meet or exceed a quote for identical service from any other US company.
ADI offers custom peptide synthesis, conjugation, antibodies, and purification in a single facility. This will not only save valuable time but it is also most economical. We can conjugate the supplied peptide to carrier proteins and make antibodies or make antibodies to a conjugate supplied by researchers. You can request only the peptide synthesis or have the peptide conjugated to carrier proteins. We are willing to offer as much help as necessary.
Custom Peptide Synthesis Only Please click over the links for more info
Custom Antibodies to Ready-to-Inject Antigens/proteins (Proteins, Peptide-Protein Conjugates, and Gels).
Custom Antibodies to Phosphopeptides
Custom Antibodies to Acetylated (lysine)
Custom Peptide Synthesis, Conjugation, and Anti-Peptide Antibodies.
Anti-Peptide Antibody package is designed to be economical and includes the following:
· Peptide synthesis (HPLC, Mass Spec, Scale 10-15 mg; 70-85% purity)
· Supply of free peptide (~ 5 mg is used for antibody production and ELISA and the rest is supplied to the researcher).
· Conjugation of peptide to carrier protein (KLH, BSA, ovalbumin, etc.).
· Antibody production in 2 rabbits (or 2 G. Pigs or 2 Chickens, extra) using std. 63 days protocol.
· ELISA titration of each antibody on first bleed.
· Approximate duration of projects (from peptide order to first bleed): 9-11 weeks depending upon the peptide length.
Peptide Synthesis and Antibody Ordering Information (Click here to open the fax order form)
Peptide Length |
Std Package |
With Peptide Upgrade |
With Affinity Purification |
With Peptide Amount Upgrade & Aff. Pur. |
Up to 20 AA |
ABPEP-20 |
ABPEP-20U |
ABPEP-20A |
ABPEP-20UA |
Up to 25 AA |
ABPEP-25 |
ABPEP-25U |
ABPEP-25A |
ABPEP-25UA |
Up to 30 AA |
ABPEP-30 |
ABPEP-30U |
ABPEP-30A |
ABPEP-30UA |
Summary of Services Included in Various Packages:
Items/ |
Std Package |
With Peptide Upgrade |
With Affinity Purification |
With Peptide Amount Upgrade & Aff. Pure |
Free Protein sequence |
Yes |
Yes |
Yes |
Yes |
Peptides |
5-10 mg total |
10-20 mg total |
5-10 mg total |
10-20 mg total |
Supply of free peptide |
5 mg |
~10-15 mg |
~5 mg |
~10 mg |
Peptide Conjugation |
Yes |
Yes |
Yes |
Yes |
Animals (2 rabbits) |
Yes |
Yes |
Yes |
Yes |
Pre-immune bleeds(2-5 ml/animal) |
Yes |
Yes |
Yes |
Yes |
Production Bleeds |
Yes |
Yes |
Yes |
Yes |
ELISATesting of 1st bleeds |
Yes |
Yes |
Yes |
Yes |
Affinity Purification (includes coupling of ~ 5 mg peptide to Sepharose, purification of 1 bleed, ELISA testing of bleeds and supply of the affinity column) |
|
|
Yes |
Yes |
Antibody Production Guarantee |
Yes |
Yes |
Yes |
Yes |
All projects include 2 rabbits, but we can substitute them with 2 G. Pig/Hamsters/or Chicken at no extra charge. Goat and sheep will incur additional charges. We can modify the std immunization and bleed protocols and include more animals if requested. Other animals, not included above, can be provided as well. Please contact ADI for current prices and availability. Std 63 days protocol include up to 5 injections (i.m. and s.c. at multiple sites at 15 days intervals); 1 preimmune bleed (~2 ml per animal), 2 immune bleeds (std. bleeds at weeks 7 and 9; ~15-20 mls per rabbit). All projects may be extended on a monthly basis, for a nominal fee, to collect more antiserum (2 bleeds per month). Please inform us prior to the conclusion of std protocol if you wish to extend the projects. Please consult Some Commonly Asked Questions About Custom Peptide And Antibody Servicesfor bleed volume in other species and optional services.
Peptide Amounts Upgrades: Standard package includes about ~10-20 mg of >90% pure peptide. This is normally sufficient to produce antibodies and have some free peptide (at least 5-10 mg) for additional experiments. However, if affinity purification (need approx. 5-10-mg peptide) is intended or needed, it is a good idea to Upgrade Amounts synthesis (synthesize 20-40 mg of 70-85% pure).
Affinity purification this package typically includes: Coupling of peptide (up to 10 mg to Sepharose), purification of any 1 bleed of ~ 15 ml or a pool of antiserum up to 15 ml; ELISA testing of both unpurified and affinity pure antibodies, and supply of affinity column We also monitor the antibody activity in the unbound fraction to make sure antibodies are not overloaded and wasted. Ideally, we need approx. ~10 mg of antigen or peptide for making the affinity matrix. This package (Cat # AFFI-1; $695) can also be ordered separately.
Note : Affinity purification is normally scheduled on 2nd bleed (pool of sera from both rabbits) unless antibody titer is poor in the first and 2nd bleed. However, user may elect to delay affinity purification or use just 1 rabbit serum. We must be informed prior to the second bleed.
ELISA: ADI uses proprietary techniques and buffer solutions to coat free peptides on ELISA plates. Therefore, antibodies to the carrier protein are not detected. We test individual preimmune and immune serum at 4 dilutions (1:100, 1:1K, and 1:10K, 1:100K). A detailed ELISA report is provided with every test regarding antibody titer of each animal. Antibody titer reported in our ELISA report refers only to anti-peptide antibodies. Complete instructions of performing ELISA and reagents used are given in ELISA Procedure.
190123A
Catalog# | Name | Product Type | Data Sheet | Size | Price |