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Custom Peptide Synthesis & Antibodies
Published references using ADI custom services
Sequence analysis & Selection of Antigenic Peptides
Custom Peptide & antibody Order forms
Custom peptide synthesis
Custom Peptide Synthesis & Antibodies
Custom Peptide conjugation to KLH & agarose
Pre-activated BSA & KLH to prepare peptide-conjugate
Custom Proteins Antibodies
Custom peptide sysnthesis & antibodies
Phosphospecific Antibodies
Acetylation specific antibodies
Chicken antibodies
Custom Affinity purification of antibodies
Pre activated agarose to prepare antibody affinity column
Pre-screeing of animals
Custom antibody modificatio (HRP, biotin coupling)
Extension of antibody projects
Custom services guarantees
IACUC, USDA License and NIH/OPRR
Removal of anti-KLH, BSA or Fusion protein antibodies
Fusion-tag Antibodies
Problem reporting & Technical help
Frequently asked questions-Peptides
Frequently asked questions Custom antibodies
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Home PageOnline Product Catalog > Custom peptides & Antibodies > Custom Peptide Synthesis & Antibodies

Alpha Diagnostic Intl. Inc. (ADI) has been providing custom peptide and antibody services for the last 20 years. Our products and services have been received and appreciate by Scientists from almost every research institution and company not only in the USA and Canada but also from highly international institution.  ADI greatly appreciates this level of confidence and we will continue to improve and excel and set even higher standards.  Most companies rely on some kind of testimonial or offer of the lowest prices, ADI offers its track record, highest quality and service, and competitive prices.

  • ADI has ~20 years of track record.

  • ADI offers the most comprehensive Protein Sequence and Structure Analyses (PSSA) not only to find antigenic peptides but also to learn a lot more about the protein structure, intracellular location, and potential functions.  There is no charge for this service.  It will be extremely hard to expect this level of expertise from other companies even for a fee.

  • ADI is the most cited company for custom peptide and antibodies. You will find “Alpha Diagnostic Intl” name in most issues of Nature, Science, Cell, J. Biol. Chem., Proc. Natl. Acad. Sci., and other top-ranked journals.  ADI has posted a list of several 100 publications to document our custom services. Our testimonials are these citations.

  • ADI offers the most efficient execution of ‘your ideas and experimentations’ with its in-house USDA and NIH/OLAW approved animal research facility.

  • ADI offers the highest level of quality and service for very competitive price.  ADI will meet or exceed a quote for identical service from any other US company.

ADI offers custom peptide synthesis, conjugation, antibodies, and purification in a single facility. This will not only save valuable time but it is also most economical. We can conjugate the supplied peptide to carrier proteins and make antibodies or make antibodies to a conjugate supplied by researchers. You can request only the peptide synthesis or have the peptide conjugated to carrier proteins. We are willing to offer as much help as necessary.

Custom Peptide Synthesis Only Please click over the links for more info

Custom Antibodies to Ready-to-Inject Antigens/proteins (Proteins, Peptide-Protein Conjugates, and Gels).

Custom Antibodies to Phosphopeptides

Custom Antibodies to Acetylated (lysine)

Custom Peptide Synthesis, Conjugation, and Anti-Peptide Antibodies.

Anti-Peptide Antibody package is designed to be economical and includes the following:

Peptide Synthesis and Antibody Ordering Information (Click here to open the fax order form)

Peptide Length

Std Package
Cat # ($$)

With Peptide Upgrade
Cat # ($$)

With Affinity Purification
Cat # ($$)

With Peptide Amount Upgrade & Aff. Pur.
Cat # ($$)

Up to 20 AA

ABPEP-20($1295)

ABPEP-20U ($1495)

ABPEP-20A ($1890)

ABPEP-20UA ($2090)

Up to 25 AA

ABPEP-25($1445)

ABPEP-25U ($1695)

ABPEP-25A ($2040)

ABPEP-25UA ($2290)

Up to 30 AA

ABPEP-30($1595)

ABPEP-30U ($1895)

ABPEP-30A ($2190)

ABPEP-30UA ($2490)

 Summary of Services Included in Various Packages:

Items/
Services

Std Package

WithPeptide Upgrade

With Affinity Purification

With Peptide Amount Upgrade & Aff. Pure

 Free Protein sequence
& antigenicity analyses

 Yes

Yes

Yes

Yes

Peptides
(70-85% pure, mass spec, hplc)

5-10 mg total

10-20 mg total

5-10 mg total

10-20 mg total

Supply of free peptide

5 mg

~10-15 mg

~ 5 mg

~10 mg

Peptide Conjugation
(couple ~ 5 mg to KLH etc)

 Yes

Yes

Yes

Yes

  Animals (2 rabbits)
& 63-days immunization program

 Yes

Yes

Yes

Yes

 Pre-immune bleeds(2-5 ml/animal)

 Yes

Yes

Yes

Yes

 Production Bleeds
(15-20 mls/animal) total 70-80 mls

 Yes

Yes

Yes

Yes

 ELISA Testing of 1st bleeds

 Yes

Yes

Yes

Yes

 Affinity Purification(includes coupling of ~ 5 mg peptide to Sepharose, purification of 1 bleed, ELISA testing of bleeds and supply of the affinity column)

 

 

 Yes

Yes

 Antibody Production Guarantee
(Minimum antibody titer by ELISA ~1K or repeat the project at no cost
(see details below)

 Yes

Yes

Yes

Yes

All projects include 2 rabbits but we can substitute them with 2 G. Pig/Hamsters/or Chicken at no extra charge. Goat and sheep will incur additional charges. We can modify the std immunization and bleed protocols and include more animals if requested. Other animals, not included above, can be provided as well. Please contact ADI for current prices and availability. Std 63 days protocol include up to 5 injections (i.m. and s.c. at multiple sites at 15 days intervals); 1 preimmune bleed (~2 ml per animal), 2 immune bleeds (std. bleeds at weeks 7 and 9; ~15-20 mls per rabbit). All projects may be extended on a monthly basis, for a nominal fee, to collect more antiserum (2 bleeds per month). Please inform us prior to the conclusion of std protocol if you wish to extend the projects. Please consult Some Commonly Asked Questions About Custom Peptide And Antibody Services for bleed volume in other species and optional services.

Peptide Amounts Upgrades: Standard package includes about ~10-20 mg of >90% pure peptide. This is normally sufficient to produce antibodies and have some free peptide (at least 5-10 mg) for additional experiments. However, if affinity purification (need approx. 5-10-mg peptide) is intended or needed, it is a good idea to Upgrade Amounts synthesis(synthesize 20-40 mg of 70-85% pure).

Affinity purification. This package typically includes: Coupling of peptide (up to 10 mg to Sepharose), purification of any 1 bleed of ~ 15 ml or a pool of antiserum up to 15 ml;ELISA testing of both unpurified and affinity pure antibodies, and supply of affinity column. We also monitor the antibody activity in the unbound fraction to make sure antibodies are not overloaded and wasted. Ideally, we need approx. ~10 mg of antigen or peptide for making the affinity matrix. This package (Cat # AFFI-1; $595) can also be ordered separately.

Note: Affinity purification is normally scheduled on 2nd bleed (pool of sera from both rabbits) unless antibody titer is poor in the first and 2nd bleed. However, user may elect to delay affinity purification or use just 1 rabbit serum. We must be informed prior to the second bleed.

ELISA: ADI uses proprietary techniques and buffer solutions to coat free peptides on ELISA plates. Therefore antibodies to the carrier protein are not detected. We test individual preimmune and immune serum at 4 dilutions (1:100, 1:1K, and 1:10K, 1:100K). A detailed ELISA report is provided with every test regarding antibody titer of each animal. Antibody titer reported in our ELISA report refers only to anti-peptide antibodies. Complete instructions of performing ELISA and reagents used are given in ELISA Procedure.

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Catalog# Product Description Product Type Data Sheet Size Price
ABPEP-30U
Custom Anti-Peptide antibodies (peptide synth. up to 30-aa, conjug., 2 rabbits, ELISA; with peptide amounts upgrade) Service PDF 1 $1,895.00
ABPEP-30UA
Custom Anti-Peptide antibodies (peptide synth. ~30-aa, conjug., 2 rabbits, ELISA; with peptide upgrade and Aff. Purif.) Service PDF 1 $2,490.00
AFFI-2
Custom Conjugation of peptides/proteins to Agarose (up to 10 mg antigen) Service PDF 1 $395.00
AFFI-I
Custom Affinity Purification of antibodies (Coupling of antigen to Agarose, Ab purification (10 ml), ELISA, and affinity column Others PDF 1 $595.00
ASCITE-1
Custom Production of Ascites in Balb/c mice (min 20 mice @$30/mice plus; $300 per cell line for cell culture) Service 1 Contact Sales
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Alpha Diagnostic Intl. Inc.| 6203 Woodlake Center | San Antonio, Texas 78244 USA
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