Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization.
11-aa-MAPS peptide (SGPSIVHRKCF) of actin (1) ; Designated (ACTB22-P or control peptide), Epitope location~ C-terminal,
The human ACTB12-P is quite conserved in various species: mouse, rat, human, chicken, frog, etc. Antibody cross-reactivity in various species has not been studied. The ACTB12-P control peptide, because of its small size, is not suitable for Western. It should be used in ELISA, dot blot or to neutralize antibody and confirm specificity of antibody. Unlabeled antibodies cat # ACBT12-M and purified human beta-actin protein (#ACTB12-C) are also available for control studies.
For research use only, not for diagnostic or therapeutic use.
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analy
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