Human beta actin (cat # ACTB16-N) was purified from platelets (>95%, mol wt ~43 kda) and used for control. For Western blot +ve control (Cat # ACTB12-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of ACTB12-C for good visibility with antibody Cat # ACTB11-S or other antibodies
The human ACTB12-P is quite conserved in various species: mouse, rat, human, chicken, frog, etc. Antibody cross-reactivity in various species has not been studied. The ACTB12-P control peptide, because of its small size, is not suitable for Western. It should be used in ELISA, dot blot or to neutralize antibody and confirm specificity of antibody. Unlabeled antibodies cat # ACBT12-M and purified human beta-actin protein (#ACTB12-C) are also available for control studies.
For research use only, not for diagnostic or therapeutic use.
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analy
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