A fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF) -¿¿convertase (TACE) (Km ~ 19 ¿M). Cleavage of this TACE substrate II occurs between the alanine and valine residues.
Useful for the screening of TACE inhibitors
Recommended usage: 50 mM Tricine buffer, pH 7.5, containing 100 mM NaCl, 10 mM CaCl2, and 1 mMZnCl2, 10 ¿M TACE Substrate II, 600 nM TACE; incubate at 27°C with mechanical shaking; reaction can be quenched with 50 mM EDTA (final concentration) ; cleavage monitored by excitation at ~ 320 nm and emission at ~ 395 nm
For research use only, not for diagnostic or
Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and A¿ formation in cultured cells (1
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