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Home Page >Online Product Catalog > 2-Antibodies & ELISA Reagents > Antibody-HRP labeling & biotinylation kits > Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP) (Kit)

Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP)

Catalog# Product Description Product Type Antibody Type Size Price
80225 Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP) Kit 1 kit $325.00



Form
Kit

Samples
user supplied proteins, peptides or antibodies

KitContents
1. 3 visal of Preactivated HRP (200 ug; lyophilized) # 80226 2. Conjugation Buffer, 0.1 M NaHCO3, pH 9.5 (10 X), 100 ml; (dilute 1:10 with water before use) # 80222 3. Blocking Buffer; 500 ul; # 80223 4. Conjugate Stabilizer; 2 ml., # 80224 5. Dialysis bag (Cut off size 10-12 kda) # DB-10 If proteins of 10kda or less are being coupled then it is a good idea to use membranes with lower cut-off such as (3 kda). Wash the membrane thoroughly with 70% ethanol and then with distilled water.

SpCrossreactivity
General purpose reagents for coupling antibodies or proteins to HRP.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.

Data Sheets
80225.pdf

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

General Information

HRP is widely used in ELISA/EIA as a detection molecule. HRP, a glycoprotein (40 kDa), is available in highly purified form, relative inexpensive, very stable, and provides several options (free amino, carboxyl, and carbohydrate groups) for covalent coup

Citations
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