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Home Page >Online Product Catalog > ELISA Kits, TruStrips and Rapid Tests > Elisa Kits for Mouse Proteins > Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative (ELISA Kit)

Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative

Catalog# Product Description Product Type Antibody Type Size Price
100-210-TNM Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative ELISA Kit 1 kit $545.00


Stds=50-1000 pg/ml; Sample=100 ul; Sensitivity= 25 pg/ml; 195 min assay; Quantitative, Sandwich, 96 tests

Serum. Plasma, Culture medium and other fluids may be adapted

1. Anti-TNFÿ¿ coated strip plate (8 wells x 12 strips) 100-2112. Mouse TNFÿ¿ Standard. A (0 pg/ml), 0.6 ml 100-2123. Mouse TNFÿ¿ Standard B (25 pg/ml), 0.6 ml 100-2134. Mouse TNFÿ¿ Standard C (100 pg/ml), 0. 6 ml 100-2145. Mouse TNFÿ¿ Standard D (250 pg/ml), 0. 6 ml 100-2156. Mouse TNFÿ¿ Standard E (500 pg/ml), 0. 6 ml 100-2167. Mouse TNFÿ¿ Standard F (1000 pg/ml), 0. 6 ml 100-2178. (20x) Sample Diluent, 10ml SD-209. Wash Buffer (100X), 10 ml WB-10010. (100x) Anti-Mouse TNFÿ¿ Detection Ab, 0.12 ml 100-21811. (100x) Streptavidin-HRP conjugate, 0.12ml S-HRP10012. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml 8010114. Instruction Manual 100-210-TNM

This kit is specific to mouse TNF alpha and does not show any significant reactivity to other Mouse serum proteins. Cross reactivity was tested with the following purified TNF-a at 100pg/ml: Rat TNF-a showed (30%) reactivity with Mouse TNF-a. Human TNF-a showed less than 1% cross-reactivity. The following serums were test with no dilution: Monkey, Hamster, Guinea Pig, Goat, Rabbit, Chicken, Sheep, Pig, FBS, and Bovine serum all showed less than 1% cross reactivity.

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.

Data Sheets

TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

General Information

TNF is a proinflammatory cytokine that provides a rapid form of host defense against infection. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is i

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