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Home Page >Online Product Catalog > ELISA Kits, TruStrips and Rapid Tests > Elisa Kits for Mouse Proteins > Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative (ELISA Kit)

Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative

Catalog# Product Description Product Type Antibody Type Size Price
100-210-TNM Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative ELISA Kit 1 kit $545.00



SpeciesReactivity
Mouse

Form
Stds=50-1000 pg/ml; Sample=100 ul; Sensitivity= 25 pg/ml; 195 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-TNFÿ¿ coated strip plate (8 wells x 12 strips) 100-2112. Mouse TNFÿ¿ Standard. A (0 pg/ml), 0.6 ml 100-2123. Mouse TNFÿ¿ Standard B (25 pg/ml), 0.6 ml 100-2134. Mouse TNFÿ¿ Standard C (100 pg/ml), 0. 6 ml 100-2145. Mouse TNFÿ¿ Standard D (250 pg/ml), 0. 6 ml 100-2156. Mouse TNFÿ¿ Standard E (500 pg/ml), 0. 6 ml 100-2167. Mouse TNFÿ¿ Standard F (1000 pg/ml), 0. 6 ml 100-2178. (20x) Sample Diluent, 10ml SD-209. Wash Buffer (100X), 10 ml WB-10010. (100x) Anti-Mouse TNFÿ¿ Detection Ab, 0.12 ml 100-21811. (100x) Streptavidin-HRP conjugate, 0.12ml S-HRP10012. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml 8010114. Instruction Manual 100-210-TNM

SpCrossreactivity
This kit is specific to mouse TNF alpha and does not show any significant reactivity to other Mouse serum proteins. Cross reactivity was tested with the following purified TNF-a at 100pg/ml: Rat TNF-a showed (30%) reactivity with Mouse TNF-a. Human TNF-a showed less than 1% cross-reactivity. The following serums were test with no dilution: Monkey, Hamster, Guinea Pig, Goat, Rabbit, Chicken, Sheep, Pig, FBS, and Bovine serum all showed less than 1% cross reactivity.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.

Data Sheets
100-210-TNM.pdf

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

General Information

TNF is a proinflammatory cytokine that provides a rapid form of host defense against infection. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is i

Citations
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