Non-immune Goat IgG Cat# 20011-1
Bacterial GST (Schistosoma japonicum, ~27 kda) was expressed in E. coli and purified (>97%). Purified GST was injected into goats to produce high titer polyclonal antibodies. Antibodies (IgG) were purified by delipidation, salt fractionation, and ion-exchange (IgG) (cat # GST13-A). It is supplied in PBS, pH 7.4 at 1 mg/ml in liquid or powder form. Dissolve in 100 ul water and use at 1:1K-1: 5K for Western and 1:1K-: 10K for ELISA.
Anti-GST IgG was coupled to agarose at 2-5 mg/ml of beads (Cat # GST13-AS). The affinity matrix is supplied in PBS pH 7.4 containing 0.05% azide. The column has a binding capacity of approx. 5 mg/ml of beads
For research use only, not for diagnostic or
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified p
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