Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the
Non-immune Rabbit IgG Cat# 20009-1
Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the General Information tan under Product overview section. Control or blocking peptid
Bacterial GST (Schistosoma japonicum, ~27 kda) was expressed in E. coli and purified (>97%).
This antiserum or IgG have been tested by ELISA (suggested dilution 1K-10K) using 50-100 ng/well GST-coated ELISA plates. It recognizes both native GST or GST fusion proteins in Western blots (suggested dilution 1K). Antibody dilution will vary depending upon the type of technique used to detect primary antibody such calorimetric or chemiluminescence substrates. The nature and concentration of GST or GST fusion proteins may also affect antibody concentration.
For research use only, not for diagnostic or
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified p
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